Description
Assignment
RECOMBINANT DNA TECHNOLOGY
Course Code: MBC-006 Maximum marks:100
Assignment code: MBC-006/TMA/2024-25
Note: Attempt all questions. The marks for each question are indicated against it.
For any question worth 2 marks, the word limit is 50 words, for 5 marks question it is 100 words; and for
10 marks it is 250-300 words.
PART-(A) Maximum marks: 50
1 Give an account of modification enzymes used for manipulating DNA in rDNA
technology.
10
2 (a) What are the characteristic features of plasmids as cloning vector? 5
(b) Explain any two vectors used for cloning large DNA fragments. 5
3 Describe TMV as a plant viral vector. 10
4 (a) What is -complementation? 5
(b) Describe digital PCR and list its applications. 5
5 Explain the Lac and Trp operon promoter and its application in rDNA technology. 10
PART-(B) Maximum marks: 50
1 Describe the applications of oligonucleotide probes. 10
2
(a)
Write a note on the following:
cDNA libraries.
5
(b) Differential mRNA display. 5
3 Describe Sanger’s di-deoxy method of DNA sequencing. 10
4 (a) What are recombinant vaccines? Explain 10
5 (a) Write a note on herbicide resistant plants. 5
(b) What are the ethical and biosafety concerns while doing rDNA experiments? 5
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