Description
ASSIGNMENT
MOLECULES OF LIFE
Course Code: BBCCT-125
Assignment Code: BBCCT-125/TMA/2025
Maximum Marks: 100
Answer all the questions given below.
1. Define Recombinant DNA Technology and describe the steps involved in its process.
(5+5) 10
2. What is the role of restriction endonucleases in genetic engineering? List and classify their types.
10
3. Compare and contrast Southern, Northern, and Western blotting techniques. What are their
applications?
10
4. Explain the mechanism of action of DNA ligase in joining sticky and blunt-ended DNA
fragments.10
5. What are the steps involved in gene cloning, and why is a cloning vector necessary? 10
6. Explain the significance of synthetic oligonucleotides in molecular cloning and the steps
involved in their synthesis. 10
7. Describe the polymerase chain reaction (PCR) and its variants. How does PCR contribute to
genetic engineering? 10
8. Discuss the principles and methods used in DNA sequencing. Why is sequencing essential in
genetic studies? 10
9. Illustrate the application of genetic engineering in medicine, focusing on the production of
recombinant human insulin. 10
10. A) How has recombinant DNA technology influenced agricultural practices? Provide examples.
5
B) Discuss the environmental and ethical implications of genetically modified organisms
(GMOs). 5
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