Description
ASSIGNMENT
MOLECULES OF LIFE
Course Code: BBCCT-125
Assignment Code: BBCCT-125/TMA/2024
Maximum Marks: 100
Answer all the questions given below. All Questions carry equal marks.
1. A) What is recombinant DNA technology? Explain the action mechanism of DNA modifying
enzymes.
B) Describe the methods involved in extraction of bacteria from the microbial culture.
(5+5) 10
2. A) Define synthetic oligonucleotides. Explain the steps involved in their synthesis. 4
B) Write a note on Yeast vectors. 6
3. What is replica plating technique? Explain various cloning vectors derived from E.coli plasimids.
10
4. Describe four gene transfer methods with suitable examples. (2.5 x 4= 10)
5. A) What do you understand by the term “blue white screening”? Explain its importance in gene
cloning. 5
B) What is DNA library? List the applications of genomic DNA libraries. 5
6. A) With a schematic diagram explain the principle involved in polymerase chain reaction. 5
B) Give a detailed account on methods used for studying PCR products. 5
7. A) Write a comparative note on Sanger’s and Maxum-Gilbert DNA sequencing methods. 5
B) Explain the importance of fusion tags in biotechnology with suitable examples. 5
8. Define site-directed mutagenesis. Describe any two methods with the help of flow charts. 10
9. A) Explain, how genetic engineering techniques are helpful in health care and agriculture. 5
B) Write a note on gene therapy. 5
10. A) Illustrate the steps involved in protein engineering. 5
B) Enlist applications of protein engineering in pharma industry and medicine.
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