Description
Assignment
GENE ORGANISATION, REPLICATION AND REPAIR
Course Code: BBCCT-117 Maximum marks:100
Assignment code: BBCCT-117/TMA/2024
Note: Attempt all questions. The marks for each question are indicated against it.
For any question worth 2 marks, the word limit is 50 words, for 5 marks question it is 100 words; and for
10 marks it is 250-300 words.
PART-(A) Maximum marks: 50
1 Explain DNA melting, Tm and its significance. 10
2 What is C-value paradox? Explain the organization of a prokaryotic gene. 10
3 What are the various functions of chromatin? How histone proteins help in
chromatin compaction?
10
4 (a) Draw and label structure and parts of chromosome. 5
(b) Explain the chemistry behind phosphodiester bond formation 5
5 Describe the enzymatic activities associated with prokaryotic DNA polymerases. 10
PART-(B) Maximum marks: 50
1 Describe the mechanism of action of topoisomerases. 10
2 Explain the process of replication in prokaryotes. 10
3 Explain the integration of λ-phage DNA into bacterial genome. 10
4 (a) Differentiate between transposons and retrotransposons 5
(b) Write a short note on chloroplast inheritance. 5
5 (a) Differentiate between spontaneous and induced mutation. 5
(b) Explain mismatch and recombinant repair mechanism for DNA repair.
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