Description
ASSIGNMENT
PROTEINS
Course Code: BBCCT-105
Assignment Code: BBCCT-105/TMA/2024
Maximum Marks: 100
Answer all the questions given below.
1. Explain the classification of amino acids based on structure and metabolic fate. 10 M
2. Write a short note on biologically active peptides. 5 M
3. With the help of schematic diagrams explain non-mechanical methods of cell disruption. 5 M
4. Discuss the protein concentrating technique that is based on freeze drying principle. 5 M
5. Write the principles of thin layer, gelfitration and affinity chromatography. Explain their
applications. 10 M
6. Give a detailed account on chromatographic technique used for the separation of volatile
compounds. 5 M
7. A) Describe the working principle of electrophoresis and list its applications. 5 M
B) Explain the N-terminal protein sequencing method 5 M
8. A) Discuss how enzymes are used for generation of protein fragments? 5 M
B) Explain the working principle of mass spectrometry 5 M
9. A) Write a short note on applications of tandem mass spectrometry. 5 M
10. A) Draw Ramachandran plot and explain it importance. 5 M
B) What is NMR? List its important applications of protein structure determination. 5M
11. Describe principles of three techniques used for protein 3-D structure analysis. 10 M
12. What is a Biological data base? Give suitable examples. 5 M
13. Give a detailed account of structural proteins with diagrams. 5 M
14. What are immune globulins? Give a short account on their classifications. 5 M
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