Description
ASSIGNMENT
Enzymes
Core Course in Biochemistry
Course Code: BBCCT-107
Assignment Code: BBCCT-107/TMA/2024
Maximum Marks: 100
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Note: Attempt all questions. The marks for each question are indicated against it. Write all
answers in your own words; do not copy from the course material.
PART-(A) (50)
1. Write in brief on the following:
a) Prosthetic Group
b) Bisubstrate Reactions
c) Enzyme Specificity
d) Mixed inhibition
(2.5*4=10)
2. a) Explain Transition state theory of enzyme catalyzed reactions. (5)
b) What is the effect of metal ions on enzyme activity? (5)
3. Give an overview of IUBMB system of enzyme classification. (10)
4. Differentiate between:
a) Ping Pong Bi-Bi and Random Sequential Mechanism
b) Kcat and Turnover Number
(5+5=10)
5. Derive Michaelis-Menten equation for a non-competitive inhibitor reactions. (10)
PART-(B) (50)
6. How compartmentation helps to control metabolic pathways? (10)
7. Write note on any two of the following:
a) Flavin nucleotides
b) Diagnostic Enzymes
c) Effect of pH on immobilized enzyme
(5+5=10)
8. Discuss the Fatty Acyl Synthase complex. (10)
9. Differentiate between the following:
i) Metal Activated enzymes and Metalloenzymes
ii) Gel Entrapment and Fibre entrapement method of enzyme immobolization
(5+5 =10)
10. Discuss application of enzymes in the following:
i) Food industry
ii) Fruit Juice and Wine industry
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